The article presents the results of comparative research of organizational culture of international cooperation departments of Russian and American Universities: University of Wyoming and Saratov State University. The purpose of the research is a detection of cultural similarities and distinctions at the level of university division leaders that are responsible for success of internationalization. This research is carried by methodology of the order diagnostics of the organizational culture at the level of leader directed to the identification of the development level of three suborders of organizational culture. According to the results of diagnostics, in the American University the dominating suborder is «family», in the Russian University – «army». Following the results of the comparative analysis, the conclusion is that in the majority of questions ethical meanings of the leaders coincide, that is a favorable factor for creation of constructive dialogue of cultures between universities.